Información, conocimiento y percepción sobre el riesgo de contraer el dengue en Argentina: dos experiencias de intervención para generar estrategias locales de control / Information, knowledge, and perception of dengue risk in Argentina: two intervention experiments to generate local control strategies

With the objectives of dengue prevention, health promotion, and action-based research, two experiments were conducted in pilot areas of Buenos Aires and Vicente López, Argentina. In each area, community, government, and non-governmental references were identified. The study included an entomological survey (with ovitraps), environmental survey (with field observations, interviews, and workshops), and social survey (using questionnaires, interviews, and meetings). Spaces for dialogue and collaboration were developed with the community, thereby spawning participatory activities and empowerment. Environmental reordering and reduction of mosquito breeding sites were promoted by: incorporation of environmental issues and vector-borne disease prevention into the school curricula; neighborhood workshops; training of environmental agents to transmit information and train peers; and planning community environmental projects. The households were visited, seeking to integrate the residents into their own community environmental diagnosis. There were significant differences between the pilot areas in knowledge and social practices, but not according to socioeconomic status. The schools and health promotion settings proved to be the main community reference for promoting healthy environmental practices.

Con objetivos de prevención de dengue, promoción de la salud e investigación-acción se desarrollaron dos experiencias en zonas piloto de Buenos Aires y Vicente López. En cada zona se identificaron referentes comunitarios, gubernamentales y no gubernamentales. Se realizó un diagnóstico entomológico (mediante ovitrampas), ambiental (con observaciones en terreno, encuestas y talleres), y social (utilizando cuestionarios, entrevistas y reuniones). Se construyeron espacios de diálogo y trabajo conjunto con la comunidad, generando acciones participativas y empoderamiento. Se fomentó el ordenamiento ambiental y la reducción de criaderos de mosquitos a través de incorporación de temas ambientales y prevención de enfermedades vectoriales a los currícula escolares; talleres vecinales de difusión; capacitación de promotoras ambientales para transmitir información y para capacitar a pares; planificación de proyectos ambientales de la comunidad. Se entró en los domicilio, buscando integrar a sus habitantes en el diagnóstico ambiental propio y comunitario. Se registraron diferencias significativas entre conocimientos y prácticas sociales, para ambas zonas piloto, pero no por nivel socioeconómico. Las escuelas y los entornos de promoción de salud resultaron ser los principales referentes comunitarios para fomentar prácticas ambientales saludables.

New record and larval habitats of Culex eduardoi (Diptera: Culicidae) in an irrigated area of Patagonia, Chubut Province, Argentina

The object of the present work was to identify the larval habitats of Culex eduardoi and to determine the microenvironmental conditions related to their presence in different artificial freshwater environments (temporary, semi-permanent, irrigation ditches, and drainage ditches) in tillable areas of Chubut Province, Argentina. This report represents the first record of Cx. eduardoi from this Province and extends its range to latitude 45°S. Immature stages of Cx. eduardoi were found in 8 out of 109 (7.3 percent) freshwater habitats and were significantly more prevalent in semi-permanent water bodies. Positive sites had significantly larger surface areas and more vegetation cover than negative sites.

Winter survival of immature instars of Mansonia indubitans Dyar & Shannon and Mansonia titillans Walker (Diptera: Culicidae), in Buenos Aires, Argentina

We conducted a whole year research on the ecology of Mansonia indubitans and Ma. titillans in Macáes Pond, Costanera Sur Reserve, Buenos Aires, Argentina. The usage of different floating plants by immature instars and their overwintering was analized. The percentage of usage of the available floating macrophytes (Pistia, Limnobium, and Salvinia) by the larvae and pupae was studied. Also, we defined positivity (P+) as the percentage of plants with immature instars for each plant genus on a monthly basis. Ma. immature instars were captured throughout the year and Pistia was the resource most commonly exploited by the mosquitoes. The percentage of fourth-instar larvae and pupae on Pistia roots with respect to total immature instars captured was assessed on a monthly and seasonal basis. The proportion of fourth-instar larvae and pupae from both species of Mansonia on water lettuce roots, showed significant differences between months and seasons. Our results suggest that the populations of Ma. indubitans and Ma. titillans in Macáes Pond, survive during winter mainly as fourth-instar larvae.

Control of Aedes aegypti with temephos in a Buenos Aires cemetery, Argentina

Avaliou-se a eficácia de um larvicida, temefós, para controlar Ae. aegypti em um cemitério de Buenos Aires, Argentina. Os criadouros reduziram de 18,4 por cento no primeiro período de estudo (nov de 1998 a maio de 1999, sem temefós) para 2,2 por cento no segundo (nov de 1999 a maio de 2000, duas aplicações), e para 0,05 por cento no terceiro (nov de 2000 a maio de 2001, cinco aplicações). As. ovitrampas com ovos diminuíram de 17 por cento no primeiro período para 5,8 por cento no segundo e para 2,9 por cento no terceiro. Os resultados sugerem que, em Buenos Aires, as populações de Ae. aegypti são altamente susceptíveis ao temefós. É recomendável seu uso para prevenir eventuais epidemias e não para o controle rotineiro.

Seasonal pattern of abundance of Aedes aegypti (Diptera: Culicidae) in Buenos Aires city, Argentina

In Buenos Aires, the most crowded city of Argentina, there is a potential risk of dengue virus transmission by the mosquito Aedes aegypti during late summer. The temporal patterns of oviposition activity and abundance of breeding sites of this vector were studied in two cemeteries of the city. Between September 1998 and August 1999, we examined 142 ovitraps weekly and a total of 18,010 water-filled containers. Both study areas showed remarkable differences in the percentages of positive ovitraps (19 percent vs 8 percent) and breeding sites (18 percent vs 1 percent), but similar temporal abundance patterns. The percentage of breeding sites was higher in summer and autumn than in spring and winter, and the percentage of positive ovitraps was higher in summer than in the other three seasons. Immatures were recorded from the first week of October to the second week of July, and oviposition activity from the third week of October until the end of April. In both cemeteries and with both methodologies the highest infestation levels were registered in March (ovitraps: 41.8 percent and 20.6 percent, breeding sites: 39.2 percent and 3.4 percent). These highest abundances took place after several months with mean temperatures above 20§C and accumulated rainfalls above 150 mm. A sharp decline in oviposition activity was observed when monthly mean temperature decreased to 16.5§C, and no eggs were found below 14.8§C. Seasonal fluctuation of Ae. aegypti abundances in mid-latitudes like Buenos Aires would allow reduction of the egg mosquito population through the elimination of containers during the coldest months, which are free of adults.

Aedes albopictus in an area of Misiones, Argentina

Until early 1998 the presence of Aedes albopictus had never been detected in Argentina. During April of the same year, few individuals of this species were recorded in 33 breeding sites found in 25 out of 161 inspected houses in the city of Eldorado, Province of Misiones. The homogeneous spatial distribution of the proliferation foci suggests the existence of a generalized infestation in this locality during the study period.

Variacion espacio-temporal del riesgo de transmision de dengue en la ciudad de Buenos Aires / Spatio-temporal variability in the transmission of dengue in Buenos Aires city

Los casos de dengue detectados en la Ciudad de Buenos Aires entre 1999 y 2000 confirmaron la posibilidad de brotes epidémicos. Para estudiar el riesgo de transmisión de dengue espacial y temporalmente se midió la actividad de su vector, el mosquito Aedes aegypti. La actividad de ovipostura del vector mostró un patrón de distribución heterogéneo en el tiempo y el espacio. Ocurrió entre octubre y mayo. En las áreas del Norte, Oeste y Sur de la ciudad (periferia) el vector fue detectado entre la primavera y el otoño en tanto que en las áreas del Este (microcentro-río) sólo en el verano. La proporción de sitios infestados varió entre los 3 períodos de estudio, pero las zonas infestadas coincidieron espacialmente. Aunque se observó variación anual y espacial de la infestación, el patrón se repitió durante los 3 años de estudio sugiriendo una dinámica estable. Las áreas de la ciudad no infestadas presentaron las mayores densidades de población y de empleados, mientras que las infestadas durante los 3 períodos presentaron las menores densidades. Esto podría representar una atenuación del riesgo, ya que no coincide la mayor densidad de habitantes con la mayor infestación. El riesgo de transmisión desde el punto de vista del vector se concentraría entre enero y marzo y ocuparía el 50% de la superficie de la ciudad en la periferia.

Ochlerotatus albifasciatus in rain pools of Buenos Aires: seasonal dynamics and relation to environmental variables

The immature stages of Ochlerotatus albifasciatus develop in temporary pools. The present study aims at evaluating the seasonal dynamics of the aquatic stages of this mosquito, also analyzing the relationship among their presence and breeding success to some relevant climatic and environmental variables in the ephemeral rain pools of an urban park. Nineteen cohorts of O. albifasciatus that developed synchronously after rain events were recorded in all seasons. The proportions of mosquito-positive pools were significantly higher during the fall-winter period than in the spring-summer months (p < 0.001). The presence of this mosquito species was positively related to the amount of rain (p < 0.001), whereas negatively correlated to air temperature (p < 0.05) within a 5.2 to 29.7ºC range. The distribution of the number of cohorts per pool throughout the year was grouped (variance/mean: 3.96), indicating that these habitats were not equally suitable as breeding sites. The immature stages of O. albifasciatus were detected in pools belonging to all of the categories of surface area, depth, duration, vegetation cover, and insolation. However, the proportion of pools where immature mosquitoes were detected was positively and significantly related to surface, depth, duration, and vegetation cover. On the other hand, the proportion of mosquito-positive pools was higher at an intermediate insolation degree. Our results suggest that although preimaginal stages were present in all seasons, high temperatures may be unfavorable to larval development, and substrate vegetation may regulate water temperature. The positive relationship between the proportion of mosquito-positive pools and pool size and duration might reflect a strategy of O. albifasciatus to accomplish immature development

Suitability of containers from different sources as breeding sites of Aedes aegypti (L) in a cemetery of Buenos Aires City, Argentina

Cemeteries are ideal urban areas to study the importance of different types of containers as breeding sites of Aedes aegypti (L.). In the present study, the suitability of plastic, glass, ceramic and metal containers was evaluated in four patches within a cemetery of Buenos Aires City, Argentina. Between October 1998 and May 2000, we found 215 breeding sites of Ae. aegypti out of 13,022 water-filled containers examined. In two patches containing microenvironments sheltered from the sun, the use of the different types of containers was proportional to the offer (correlation coefficient = 0.99, P < 0.05 in both cases). In the remaining patches, plastic and metal containers were the most and less frequent breeding sites, respectively (P < 0.001 in both cases). The number of immatures per breeding site (median = 4.5) did not show significant differences among the four types of containers examined (H3, 215 = 1.216, P = 0.749). Differences found in patches from a same cemetery suggest that different microenvironmental conditions affect the suitability of each type of container for Ae. aegypti breeding. Plastic containers appeared as key breeding sites that should be removed to reduce the Ae. aegypti population in the study area

Characterization of Triatoma guasayana biotopes in a hardwood forest of Santiago del Estero, Argentina

Triatoma guasayana is a silvatic triatomine species distributed in Argentina, Bolivia and Paraguay. The study was performed in a secondary forest of Santiago del Estero, Argentina. The abundance of T. guasayana was evaluated by census in the following wild biotopes: quimiles (Opuntia quimilo), chaguares (dry bromeliads), logs and underground burrows. Ten biotopes of each type were dismantled in winter (August) and another 40 in summer (January); all fauna was recorded. The biotopes most infested by T. guasayana were quimiles (65 percent), followed by chaguares (55 percent), and logs (25 percent). Quimiles and chaguares were infested in both seasons, whereas logs were positive only in summer and burrows were never infested. Infestation and abundance were higher in summer than in winter. The biotope structure is a key factor for T. guasayana colonization. The larger number of refuges, the constant presence of blood sources and suitable inner microclimatic conditions offered by quimiles may favour the persistence of T. guasayana colonies. The richness of invertebrate fauna per type of biotope was ranked in the same order as that of T. guasayana, suggesting similar microhabitat requirements for all studied arthropods

Environmental characteristics of the cemeteries of Buenos Aires City (Argentina) and infestation levels of Aedes aegypti (Diptera: Culicidae)

Cemeteries with many water-filled containers, flowers, sources of human blood, and shade are favorable urban habitats for the proliferation of Aedes aegypti, a vector of yellow fever and dengue. A total of 22,956 containers was examined in the five cemeteries of the city of Buenos Aires, Argentina. The vector was found in four cemeteries that showed an average infestation level of 5.5 percent (617 positive out of 11,196 water-filled containers). The four cemeteries positive for Ae. aegypti showed significantly different (p<0.01) infestation levels. Vegetation cover and percentage of infestation were significantly correlated (p<0.01), but neither cemetery area nor number of available containers were significantly related to the proportion of positive vases. Our results suggest that the cemeteries of Buenos Aires represent a gradient of habitat favorableness for this vector species, some of which may act as foci for its proliferation and dispersal

Effects of flooding and temperature on Aedes albifasciatus development time and larval density in two rain pools at Buenos Aires University City

Aedes albifasciatus is a floodwater mosquito that breeds in temporary waters. This semi-domestic species, widely distributed in Argentina, is a competent vector of the western equine encephalitis. The present study was carried out in two rain pools of the city of Buenos Aires, from April 1998 through March 1999. Samples were taken twice a week during the cold season and daily during the warmer months, starting from October. Immature mosquitoes were collected with a dipper, being the number of dippers proportional to the flooded area. The estimated rainfall thresholds to initiate cohorts of Ae. albifasciatus were: 16-17 mm in the fall-winter period, 25 mm in the spring, and 30 mm in the summer. The development time of the different cohorts and the mean air temperature of their respective periods were estimated in all seasons, ranging from six days (at 24o.C) to 32 days (at 13o.C). The equation that best expresses the relationship between development time and mean air temperature is dt =166,27.e-0,1435.T (R2=0,92). Significantly shorter development times were recorded for larvae of the first three stages as compared to the fourth larval stage and pupae

Contaminación por helmintos en espacios públicos de la provincia de Chubut, Argentina / Contamination by helminths in public places at Chubut province, Argentina

Canine-borne helminthiases constitute a worldwide problem. Some of these parasites can originate serious pathologies in humans. Given the potential existence of these zoonoses in Argentina, horizontal surveys on contamination degree evaluated as faeces per hectare and on parasite prevelence in faeces were carried out in six localities of the Province of Chubut. The behaviour of people visiting public places was also analyzed qualitatively. All of the survey sites were contaminated with faeces and with some parasitic forms. Contamination degrees ranged from 32 to 147 faeces/ha. Toxocara sp. (17.4 percent), Strongyloides sp. (5.1 percent), and Ancylostoma. sp (5.6 percent) were the main genera among other parasites detected. As a whole, the analysis revealed that contamintion degree, prevalence and human behaviour in relation to the exposure to the parasite would be the main factors to be taken into a account in formulating control measures. Transmission risk patterns in Chubut were not homogenous; therefore, studies and control strategies should be devised and implemented at the local scale

Estudio de la prevalencia de la infección por Trypanosoma cruzi en zarigueyas (Didelphis albiventris) en Santiago del Estero, Argentina / Study of the prevalence of infection by Trypanosoma cruzi in opossums (Didelphis albiventris) in Santiago del Estero, Argentina

Entre los principales reservorios silvestres de Trypanosoma cruzi se encuentran las zarigueyas del género Didelphis, ampliamente distribuidas por el continente americano. En Amamá y Trinidad, Provincia de Santiago del Estero, Argentina, Didelphis albiventris es el marsupial más frecuente. Su población se renueva cada año y normalmente hay dos períodos reproductivos: uno a principios de la primavera y otro a principios del verano. Estas dos camadas son destetadas y abandonan la bolsa marsupial para incorporarse a la población, la primera (G1) a principios del verano y la segunda (G2) a principios del otoño. Entre 1988 y 1991 se estudiaron 409 individuos distintos de D. albiventris y los xenodiagnósticos revelaron que 35 por ciento de ellos estaban infectados por T.cruzi. Se observaron ciclos de renovación anual de la infección con prevalencias que oscilaron entre 22 y 43 por ciento. La adquisición del parásito ocurría a lo largo de todo el año, desde el verano hasta la primavera. La prevalencia de la infección aumentó con la edad. Los individuos G1 tuvieron tendencia a presentar mayores prevalencias que los G2, probablemente debido a un mayor tiempo de exposición a la transmisión. En las dos primeras categorías de edad, los individuos G2 mostraron mayores prevalencias que los G1, lo cual indica un aumento significativo de la intensidad de la transmisión durante el otoño. Las zarigueyas deberían considerarse como una fuente potencial de ingreso de T.cruzi al ciclo doméstico

Interaction between Didelphis albiventris and Triatoma infestans in relation to Trypanosoma cruzi transmission

This paper attempts to prove if a high Trypanosoma cruzi prevalence of opossums might be reached with few potential infective contacts. One non-infected Didelphis albiventris to T. cruzi and 10 infected nymphs of Triatoma infestans were left together during 23 hr in a device that simulated a natural opossum burrow. Twenty-six replicates were perfomed using marsupials and triatomines only once. Potentially infective contacts occurred in all the trials. From the 26 opossums used in trials, 54 por cento did not eat any bug. Of the 260 bugs used, 21 por cento were predated. In the 25 trials involving 205 surving bugs, 36 por cento of them did not feed. In 15/25 cases, maior ou igual a 60 por cento of the triatomines were able to feed. The parasitological follow-up of 24 opossums showed that among 10 that had eaten bugs, 4 turned out infected and among the 14 that had not predate, 3 (21 por cento) became positive. In sum, 7/24 (29 por cento) of the marsupials acquired the infection after the experiment. This infection rate was similar to the prevalences found for the opossum population of Santiago del Estero, Argentina, suggesting that the prevalences observed in the field might be reached if each marsupial would encounter infected bugs just once in its lifetime.

The interaction between poultry and Triatoma infestans Klug, 1834 (Hemiptera: Reduviidae) in an experimental model

This paper compares the predation pressure that ducks and chickens exert on triatomines. For the tests, these birds were placed in individual boxes together with a known number of Triatoma infestans and left to interact from 6 p.m. till the next morning, involving a long lasting period of complete darkness limited by two short-term periods of semi-darkness. There was a shelter which could prevent the bugs from being predated. The number of live and dead triatomines was recorded, considering missing bugs as predated by the birds. Ducks exhibited a greater predatory activity than chickens, that could be due to a long term active period at night while chickens sleep motionless from sunset to dawn. Surviving triatomines that had fed on chickens outnumbered those fed on ducks suggesting that these were less accessible to the triatomine biting. If ducks are better than chickens to detect and eat bugs and to interfere with their feeding in the field, an increase in duck number might help to diminish triatomine density. Further research is needed to determine the feasibility of application of these experimental results.

Dispersive flight and house invasion by Triatoma guasayana and Triatoma sordida in Argentina

Flight activity and invasion of houses by Triatoma sordida and T. guasayana were studied in the Province of Santiago del Estero, Argentina. Spontaneous findings of both species in houses were recorded from 1982 to 1989. Light trap collections were performed in 1982, 1983 and 1984, at the woods surrounding the settlements of Amamá (43 houses) and Trinidad (19 houses). Most of the 101 triatomines collected, were unfed and negative for Trypanosoma cruzi. T. guasayana predominated over T. sordida, and both appeared on the lighted screens between 19-31 min (mean 24) after dusk and the catch time was 30-45 min. Although entomological evaluation of 41 houses at Amamá performed in September 1985, just before insecticidal spraying, showed that Triatoma infestans predominated, adults of T. guasayana were collected in sleeping places, in 7 houses (17%). Most triatomines invading houses from then up to 1990 were flying T. guasayana (20/27) and females outnumbered males. Three non infected T. guasayana females were fed on man and two T. guasayana males positive for "T. cruzi like" trypanosomes were unfed. Therefore, visiting hungry adults could transmit T. cruzi to people and introduce wild parasites to the domestic cycle. T. guasayana stands as the main potential substitute of T. infestans in the studied area, and it might play there the same role as T. sordida in Brazil